Insertion junction: CLIP2.077706_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.077706
Chromosome:chromosome 16
Location:5413331
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g682800 3'UTR
Cre16.g682850 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAGTTTCAACGTCTTCGGCCAGACATTGCACCAACATGGCGTGGCGGCA
Internal bar code:CATCGTAATTTTCATTGGGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:811
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAGGATGATGACGACCTG
Suggested primer 2:GCGCAGACATGTTGGTTTGT