| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.077712 |
| Chromosome: | chromosome 14 |
| Location: | 943775 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613501 | ZXE5 | (1 of 9) PTHR13789//PTHR13789:SF186 - MONOOXYGENASE // SUBFAMILY NOT NAMED; Putative zeaxanthin epoxidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCACGGAGCTTTAAGACTTTTCCTCTCGACTACCCCATCCCCGCTCCG |
| Internal bar code: | TGTGTAGCCTCTAGTATAGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1570 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAAGGTTGCTCTCACCGT |
| Suggested primer 2: | TCCCAACAACCCTCTGCATC |