| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077718 |
| Chromosome: | chromosome 6 |
| Location: | 3216109 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g276600 | HTR2 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCTTGCGGATCAGCAGCTCAGTGGACTTCTGGTACTTGCGAATCTCG |
| Internal bar code: | TGCCATCCGGCTCACCGTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 357 |
| LEAP-Seq percent confirming: | 44.4444 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGTGCCTTGTTTCCCCTC |
| Suggested primer 2: | TCTGGCTGTGATTGAGCAGG |