Insertion junction: CLIP2.077732_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.077732
Chromosome:plastome
Location:126674
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802316 ChreCp052,2717043,ycf10,cemA (1 of 1) PTHR33650:SF2 - CHLOROPLAST ENVELOPE MEMBRANE PROTEIN; heme-binding protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAACTACTATCACGTTTACTCACAAATGGAACAATATTTACACCTCTTTG
Internal bar code:CCGGGGTGAATTGTGGCTGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:128
LEAP-Seq percent confirming:55.5556
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AATGACCCTGCTCGTAACCC
Suggested primer 2:ATTTCACCCTCTGCGGAAGG