Insertion junction: CLIP2.077741_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.077741
Chromosome:chromosome 10
Location:2886616
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g439650 COG8 (1 of 1) PF04124 - Dor1-like family (Dor1); Component of oligomeric golgi complex 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGATGCCGCATGCCGTGATGCCAACTGTCTGTCACTGTGATTGGGCGCTG
Internal bar code:ACTGCGCATGATGACCGTAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1355
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTAGTTCGGGGCTACGGGTA
Suggested primer 2:CTCCGACACACCTTGCTCAT