| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077741 |
| Chromosome: | chromosome 10 |
| Location: | 2886621 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439650 | COG8 | (1 of 1) PF04124 - Dor1-like family (Dor1); Component of oligomeric golgi complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACGCGCGGTGCCTGCTGTTTAAGATGTGCATCTGATGGCACGCGGGCA |
| Internal bar code: | ACTGCGCATGATGACCGTAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2527 |
| LEAP-Seq percent confirming: | 80.7692 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGACACACCTTGCTCAT |
| Suggested primer 2: | TTAGTTCGGGGCTACGGGTA |