| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077754 |
| Chromosome: | chromosome 9 |
| Location: | 1024410 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402150 | (1 of 1) IPR002734//IPR024072 - Bacterial bifunctional deaminase-reductase, C-terminal // Dihydrofolate reductase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTGCAAGCGCTGAGCCTCGCCAACGTGTGGCCCTCATCACGCGACCC |
| Internal bar code: | GTGTGGATAATGAAGGGAAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 100 |
| LEAP-Seq percent confirming: | 22.2222 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCTTCTGCAACGCATCG |
| Suggested primer 2: | CGCTGCAAATATCACGTCCG |