| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077756 |
| Chromosome: | chromosome 16 |
| Location: | 7625837 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684827 | (1 of 4) PTHR11207//PTHR11207:SF0 - RIBONUCLEASE III // RIBONUCLEASE 3 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTCCTGTTCTTATGGTTCCCCAATGCTGGATAACTCTCATAGAGCACT |
| Internal bar code: | ATTGATACCATGTGTGTGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1056 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGACTGTGAGGGTGCATG |
| Suggested primer 2: | GAAACACGCCAACCTTTCCC |