| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077772 |
| Chromosome: | chromosome 1 |
| Location: | 7418484 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g052300 | E2F1 | (1 of 1) K06620 - transcription factor E2F3 (E2F3); Transcription factor, E2F and DP-related | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGAATACTGTCATTTCAATATTACACGGCAAAGTGTCTGGCAAGCCGC |
| Internal bar code: | TGGTGTAGGGAGCAAGGTCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2654 |
| LEAP-Seq percent confirming: | 98.3871 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAAATCGGTTGCTCCCAG |
| Suggested primer 2: | GCGATTGGTGGGGTACTCAA |