| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077775 |
| Chromosome: | chromosome 4 |
| Location: | 309426 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g800432 | FTSHi2,CTAP6,FTSHI2 | (1 of 3) IPR000642//IPR003593//IPR003959//IPR027417 - Peptidase M41 // AAA+ ATPase domain // ATPase, AAA-type, core // P-loop containing nucleoside triphosphate hydrolase; Chloroplast-import FtsH-like ATPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATTGAATGGGTTTCCTGCAAGACGCGCAGCGGTGTGTGTGTGTGCGGG |
| Internal bar code: | GGCATCATGATTCTGCTCAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1006 |
| LEAP-Seq percent confirming: | 85.3659 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGCTGCCATGATTCTGA |
| Suggested primer 2: | GAGTGAGCGGACCATATGGG |