Insertion junction: CLIP2.077778_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.077778
Chromosome:chromosome 6
Location:8380047
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g307800 CYN49 (1 of 1) IPR003609//IPR029000 - PAN/Apple domain // Cyclophilin-like domain; Cyclophilin 49 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGCACACCTGGCACACACACACATCCGCGCGCCCTCGCGCCAATGCCG
Internal bar code:GTCTGAATGAAATCGGAATAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:387
LEAP-Seq percent confirming:20.9302
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:CACAAACGCGTGTTCGAACT
Suggested primer 2:GACAGTTTGGCACACACGAC