Insertion junction: CLIP2.077793_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.077793
Chromosome:chromosome 6
Location:5355589
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g285050 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGCCTGGCATGTTGCACACTTGTACTCATCGCATCCGAAGGCCCTCCC
Internal bar code:ACTCCGAAAAACTGGTGGACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1122
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAATTTCAGGACCAGCCG
Suggested primer 2:GCGTCGTATGTTCCATTGGC