| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.077810 |
| Chromosome: | chromosome 14 |
| Location: | 1224746 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615850 | EFH8 | EF-hand Calcium binding protein; (1 of 38) IPR002048//IPR011992 - EF-hand domain // EF-hand domain pair | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGATGAGGAGAGAGTACTCCACGCACGCTCTAAGTAAGCGACAGTTGCG |
| Internal bar code: | AAGACCCCATCGTTTCTGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1279 |
| LEAP-Seq percent confirming: | 88.4615 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAACGCGATGGAAAAGTCC |
| Suggested primer 2: | GGTGAGTGAAAGTGAGCGGA |