| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077812 |
| Chromosome: | chromosome 12 |
| Location: | 2627233 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505900 | TIM50 | (1 of 1) K17496 - mitochondrial import inner membrane translocase subunit TIM50 (TIM50) | 3'UTR |
| Cre12.g505950 | TIM50 | (1 of 1) PTHR12210//PTHR12210:SF3 - NUCLEAR LIM INTERACTOR-INTERACTING FACTOR-RELATED // MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM50; Mitochondrial inner membrane translocase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGCACGCACGCATCCGCACTCCTCTTCCTGCCTTTCAAAATGGGGCC |
| Internal bar code: | CTATTGGATTTGCTTAGGTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3543 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACATACGGTATGGCGCCA |
| Suggested primer 2: | CTTCACGATCGACACCAGCT |