Insertion junction: CLIP2.077841_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.077841
Chromosome:chromosome 7
Location:5664204
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g352600 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCCTTTGCCTCGCCTCACTGCACCTTTGTCATTTTTCTTAAAGCTTAT
Internal bar code:GTCCGCTGGGCTGATTGTAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:51
LEAP-Seq percent confirming:5.55556
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:ATGATGGTGTTGGTGGCGAT
Suggested primer 2:GCTGCAATGTTGACCAGAGC