| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.077841 |
| Chromosome: | chromosome 7 |
| Location: | 5664233 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352600 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCCCCAGCACTTGTATCCGCCGCTAGAGCCGATGCAGACCTAAAGGC |
| Internal bar code: | GTCCGCTGGGCTGATTGTAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 27.2727 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCAATGTTGACCAGAGC |
| Suggested primer 2: | ATGATGGTGTTGGTGGCGAT |