Insertion junction: CLIP2.077854_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.077854
Chromosome:chromosome 9
Location:634221
Confidence (%):40
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGTGGCTGGGTGACGGCTCAGTGGGGCTCAGTGGGGCTCAGTCGTGGG
Internal bar code:AGCTGGTTATGAGCGCAACAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:390
LEAP-Seq percent confirming:10.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCACAGTTGTCAACAGCC
Suggested primer 2:GTGGTGTACGTGGACGAGTT