| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077856 |
| Chromosome: | chromosome 17 |
| Location: | 135086 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g696800 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAGACTTTAGCAAAATGTAATAATGTCAAAAAATTTTAACCTCTCAGA |
| Internal bar code: | ATTAAGGTTGGAAGTGTCGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3374 |
| LEAP-Seq percent confirming: | 76.8421 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 95 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTGATACGGGTATGCTGC |
| Suggested primer 2: | CCTCAACGCCATGTAACCCT |