| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.077877 |
| Chromosome: | chromosome 10 |
| Location: | 3530318 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCACCATTTCGTGCTCGCTGACCATTGCTTCTTGGCGAGTGCTTGTAC |
| Internal bar code: | TGAGCATTTAGCGACCGCGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 706 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCAGCCAAACCCTTGTCA |
| Suggested primer 2: | CCCACGACCGTATCCTGATG |