Insertion junction: CLIP2.077899_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.077899
Chromosome:chromosome 9
Location:367948
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCACAAACTGCCACACTCCCACGTGCTGCACCTAGCGCCCGCCCCTAGC
Internal bar code:CAGCCTGGAGGGAATTTCGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1571
LEAP-Seq percent confirming:62.5
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:AAACACGCACGAAACGACTG
Suggested primer 2:CAGTCTAAGGACCGGCGATC