Insertion junction: CLIP2.077904_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.077904
Chromosome:chromosome 7
Location:938191
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre07.g319050 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCACCATCAACACCAACGCACTCCTTACCAATATCATTCGCCCGCGTA
Internal bar code:GCGGAATGGCGGGGCGTTTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:871
LEAP-Seq percent confirming:5.88235
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGTCACTCAACCCCATGT
Suggested primer 2:GAGTGTGACAGGTGAGGTGG