| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077913 |
| Chromosome: | chromosome 5 |
| Location: | 2872324 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241500 | MOT30 | Predicted protein | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTTAGCGCGGCCGCAGCCATACACGGGTAGGTACGCGCCCAACCGCTT |
| Internal bar code: | AATTGCTGTCAGCGGACAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2325 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAGTTCACTGTGGCTTG |
| Suggested primer 2: | ATGCGCTCACCTGTGTGTAA |