| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.077928 |
| Chromosome: | chromosome 8 |
| Location: | 1158163 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g363050 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCTGCCAGGGCCTGGTGGAGAGCAAGGGCTGGAAGGAGGGGCTGCTG |
| Internal bar code: | AACATGACGGGGCCGACCAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 48 |
| LEAP-Seq percent confirming: | 8.33333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGAGGAGAGCAGGCAGGT |
| Suggested primer 2: | TGGATACCCCGGTTCCAGAT |