Insertion junction: CLIP2.077930_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.077930
Chromosome:chromosome 8
Location:3036852
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre08.g375200 (1 of 1) K07870 - Ras homolog gene family, member T1 (RHOT1, ARHT1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTGCTGCAAGGCCGACCGCCTCAGCGACCGCGACAGCCCCAGCATCCG
Internal bar code:CGTGTTAAGGTCCGCATCTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:335
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTGGACGAGCAGTTGAGG
Suggested primer 2:GTGCTCGCTGCTTCTTATGC