Insertion junction: CLIP2.077931_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.077931
Chromosome:plastome
Location:80276
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802297 psbB,2717002,ChreCp032 (1 of 1) K02704 - photosystem II CP47 chlorophyll apoprotein (psbB); photosystem II P680 chlorophyll A apoprotein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGGTGGTATTGCTTCACACCACATCGCAGCTGGTATTTTAGGTGTTT
Internal bar code:TTTATCATGGGTGAACAAAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:351
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTGGCATACGACGAACGA
Suggested primer 2:GTTGGGCAGGTTCTATGGCT