| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077942 |
| Chromosome: | chromosome 16 |
| Location: | 7692229 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686173 | (1 of 1) PTHR11071:SF276 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAAATTACAAACTTTGTAAGGAACTCAGCATCAAGTCCGGTCCGGTTT |
| Internal bar code: | GAGCATCATACTGGAACGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2309 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTTGGAACCGTGAAACC |
| Suggested primer 2: | CCACCGCAATGTCCATGAAC |