| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077955 |
| Chromosome: | chromosome 12 |
| Location: | 3514263 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496800 | NDUFAF3 | NADH:ubiquinone oxidoreductase 1 alpha subcomplex assembly factor 3; (1 of 1) K09008 - NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 (NDUFAF3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCATCAGACCCTCCCGTGGAAACGCGAGGCACCATTCTTTCACCAGT |
| Internal bar code: | AGAATGTTTCTACCTTCCGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 722 |
| LEAP-Seq percent confirming: | 8.33333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAGAAAGGGCAGCTTCT |
| Suggested primer 2: | GCTTCGCTGTCACACGTTTT |