| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.077960 |
| Chromosome: | chromosome 9 |
| Location: | 66072 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386161 | (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTGGCCTCAGACTGTGGCGGGGCTCAGCCCCTTTTCCCGTGACCGGG |
| Internal bar code: | GATCCTGTATGAGTTGGGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 27.4194 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGGATGCCCTAGGATGC |
| Suggested primer 2: | ACTCACTCTACCCTCCCGAC |