| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.077981 |
| Chromosome: | chromosome 6 |
| Location: | 3187687 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g276200 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGTACACCAACACTTCCCCATGGATGCTCCGTCCACACAGCGCTCCAT |
| Internal bar code: | ATACTGGCAGAAATCAGCAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4791 |
| LEAP-Seq percent confirming: | 2.38095 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACCTGTCTGTTGGGGAAG |
| Suggested primer 2: | CAAACCTGTGCCAGCAACTC |