| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077987 |
| Chromosome: | chromosome 10 |
| Location: | 6231955 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463150 | DCI1 | Enoyl-CoA hydratase/isomerase family; (1 of 1) PTHR11941//PTHR11941:SF43 - ENOYL-COA HYDRATASE-RELATED // DELTA(3,5)-DELTA(2,4)-DIENOYL-COA ISOMERASE, MITOCHONDRIAL | 5'UTR |
| Cre10.g463200 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTCATCTTTCAAGTCCATGCTGTAGGCCTACCAGAAACACAACTTGAC |
| Internal bar code: | GCTGGTCAGCAACAAGCATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1117 |
| LEAP-Seq percent confirming: | 87.2727 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGCACAGGTTCGGTTTG |
| Suggested primer 2: | GGTTTGGAGTCGAGCTGGAA |