| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077995 |
| Chromosome: | chromosome 6 |
| Location: | 965480 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g256500 | (1 of 2) PTHR14187//PTHR14187:SF5 - ALPHA KINASE/ELONGATION FACTOR 2 KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAATACGAGCGGTTCGGGTTTGGGCCTGCACTGGGAACGAAGAATGCGT |
| Internal bar code: | GGTGGAGGAGGAGGGTAGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2456 |
| LEAP-Seq percent confirming: | 18.1818 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACGCATTGTGGCCAAGA |
| Suggested primer 2: | TGTGCATTTGATTGTGCGGG |