| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.078050 |
| Chromosome: | chromosome 4 |
| Location: | 3017175 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225050 | (1 of 3) PF16166 - Chloroplast import apparatus Tic20-like (TIC20) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATTTGTTCGGCAACTGCAGTAATGGAAGCAGAAGCTGCATGGGGCATG |
| Internal bar code: | ACTATAGCGTGAGTGGCTAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2286 |
| LEAP-Seq percent confirming: | 65.3846 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGGAGTGTGGCGTTCATC |
| Suggested primer 2: | GCTGAGAGCTGGGGAATGTT |