| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.078059 |
| Chromosome: | chromosome 1 |
| Location: | 7745754 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g054550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCTATACATAGCTAGCCAACAAATGAACGCGTCTGGGTGAGGGCGAGA |
| Internal bar code: | CTGCGGGAAAGTTTTGGATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2332 |
| LEAP-Seq percent confirming: | 59.596 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 99 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAAGTTTGGCACCAGTG |
| Suggested primer 2: | TGGCTCTTGCAATCCGTCAT |