| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.078078 |
| Chromosome: | chromosome 5 |
| Location: | 1685365 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g800544 | (1 of 30) PF00078//PF03372 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) // Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGAGTGCTGGGACGGGGACGCATACGACTGCAGTTCTCTCGCCGGCGT |
| Internal bar code: | GGGCCTAATGTGTAGCCACGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 118 |
| LEAP-Seq percent confirming: | 90.625 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCCCTGCAAGCTAAGAC |
| Suggested primer 2: | CACTCCAGGAAGATGTGCGT |