Insertion junction: CLIP2.078087_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.078087
Chromosome:chromosome 6
Location:4277846
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre06.g278256 RPN8 (1 of 1) K03038 - 26S proteasome regulatory subunit N8 (PSMD7, RPN8); 26S proteasome regulatory subunit CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTGAGTGCCAGCAGCACGGCAGGTGGACAGGCGGGCCGTGGCTTTGCC
Internal bar code:GTCGTGTAAAACCGCCTAGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:176
LEAP-Seq percent confirming:12.5
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TGGAACACATACACCCAGGC
Suggested primer 2:ACCTGGACATCAACGAGCTG