Insertion junction: CLIP2.078096_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.078096
Chromosome:chromosome 2
Location:7197787
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g390763 RAB2,FAP354 Rab-like GTP-Binding Flagellar Associated Protein 354; (1 of 1) K07877 - Ras-related protein Rab-2A (RAB2A) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACACTCCGAACAAGTACTTCTCCTTGGTTTGAGATACGCTGGATTCCTT
Internal bar code:GTAGCGGTAATGGGCGTACTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3761
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTCAGCGCACGTAATTCG
Suggested primer 2:TGGCATAGGAGGTCTCTGCT