| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.078115 |
| Chromosome: | chromosome 16 |
| Location: | 2707195 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661850 | (1 of 2) PF08332 - Calcium/calmodulin dependent protein kinase II association domain (CaMKII_AD) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTCTACTTCAATGCATTGATGGGGCCGGCGAGCGACTAGGCAATCTC |
| Internal bar code: | TTTCTTTATCCCGCTTCCGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 45.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCTTGGAGTTGAGCGTCT |
| Suggested primer 2: | CTCGTGCTTCCTTGTCAGGT |