Insertion junction: CLIP2.078120_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.078120
Chromosome:chromosome 3
Location:8261635
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre03.g199700 PGM11 (1 of 18) PF00300 - Histidine phosphatase superfamily (branch 1) (His_Phos_1); Phosphoglycerate mutase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACGTAGTGTCACACCAACACCATACACCCTCAGACCCTCGGTCCAAGG
Internal bar code:ATTTGACATAGTAATGGATATC

Confirmation - LEAP-Seq

LEAP-Seq distance:663
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGCAGTACTGGTGGTGAC
Suggested primer 2:ACACGAGCTGGAAAACGGAT