| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078121 |
| Chromosome: | chromosome 17 |
| Location: | 6529602 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g744797 | (1 of 1) PF05623 - Protein of unknown function (DUF789) (DUF789) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGTATGTGCTTATTTGGGTGGGTTGCACTCAAGGGATACAAACCTTAC |
| Internal bar code: | TTGTGGAACTCTAATGTGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1480 |
| LEAP-Seq percent confirming: | 90.411 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTTCGTTTTGCGCACGA |
| Suggested primer 2: | GCGCGAGATAACAGCACTTG |