| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.078140 |
| Chromosome: | chromosome 7 |
| Location: | 2798410 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331650 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGATACCGGCATAGCGATCCACCAGCAGCGCACCTGTTTAGAGGCAG |
| Internal bar code: | GCGGTAACGTCAACTGGGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2209 |
| LEAP-Seq percent confirming: | 48.0769 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGAGCAGTGTAAGGAGG |
| Suggested primer 2: | CCATACTCATCCCGCCCTTC |