| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078144 |
| Chromosome: | chromosome 2 |
| Location: | 2272505 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g800194 | (1 of 2) PF14753 - Domain of unknown function (DUF4475) (DUF4475) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTCGGCCCTGCAGCACCCGCACCAGCACCAGCCGTGCCCGCTGGCCC |
| Internal bar code: | CGTGCTGAGGATGGATCACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 173 |
| LEAP-Seq percent confirming: | 10.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGGTTGACCCGAATGAC |
| Suggested primer 2: | GGCTGGCTACAATGGTCAGT |