| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078156 |
| Chromosome: | chromosome 7 |
| Location: | 2730220 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331200 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACACACCTCCAGCGGCGACACGGGCAGCACCTCTGAGTTGAGCATGCGC |
| Internal bar code: | CAGACTGACCGCCTGAAAAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 424 |
| LEAP-Seq percent confirming: | 68.4211 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCGCAAGCACTCTGGA |
| Suggested primer 2: | CCACTCCCACTGAGCTCTTG |