| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.078157 |
| Chromosome: | plastome |
| Location: | 130854 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802318 | ChreCp054,2716983,atpF | ATP synthase CF0 B subunit; (1 of 1) PTHR34264:SF3 - ATP SYNTHASE SUBUNIT B, CHLOROPLASTIC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTTTATTTAAACGTTCACGAACACGTGTCATAATTTTATTAATAACG |
| Internal bar code: | CGCGTGTGACGAACACAATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 49 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCAGTGGCGGTACTACT |
| Suggested primer 2: | TACTAGGACGTCCCCTTCGG |