| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.078164 |
| Chromosome: | chromosome 6 |
| Location: | 3184540 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g276200 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTGGGAAAGGCAGTCCCCGACCAGGTCGCAAAATCCGACCTCGGCTT |
| Internal bar code: | CTGCCTCGACCGATCATTCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3521 |
| LEAP-Seq percent confirming: | 91.0112 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGGTATGATTGGTGTGCC |
| Suggested primer 2: | CATCCTACGCCATTTGCACG |