| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078173 |
| Chromosome: | chromosome 9 |
| Location: | 5534738 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409300 | (1 of 5) PF06863 - Protein of unknown function (DUF1254) (DUF1254) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGGGCGGCCGGCGACGCACTCGGATTGCTCATGTACGTTGCTGTGGT |
| Internal bar code: | CTCGTCGGATCTTTGGAAGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 255 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTGAATCCGCCCGCATGA |
| Suggested primer 2: | CCGCTTCCTAGCTTTCCCAA |