| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.078176 |
| Chromosome: | chromosome 14 |
| Location: | 1216939 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615800 | (1 of 3) PTHR31563:SF1 - ION CHANNEL POLLUX-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCTGGTTTGGGCCAGGGCACGTCACGTGGTGCCAACTGAAGTGCCACG |
| Internal bar code: | GCACGAGTGAGATACACAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 337 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACCGCCCATAACCCTCC |
| Suggested primer 2: | ACCTCTACATGTTGCCCTGC |