Insertion junction: CLIP2.078187_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.078187
Chromosome:chromosome 6
Location:3117232
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre06.g275450 (1 of 1) PF00069//PF13855 - Protein kinase domain (Pkinase) // Leucine rich repeat (LRR_8) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCAGGTGCTGCTGGCGCCGCGCAACAAGCTCACGGCGCTGCCTGATGC
Internal bar code:AGAGTCCGTGACTTTGTTGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:689
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGAGAAGGAAGCTTGGGT
Suggested primer 2:GATCCAGCTTCATCCGAGGG