Insertion junction: CLIP2.078189_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.078189
Chromosome:chromosome 6
Location:5557435
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g287100 CAM11 Calmodulin-like protein; (1 of 2) PF13499//PF13833 - EF-hand domain pair (EF-hand_7) // EF-hand domain pair (EF-hand_8) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTCTTAGTTACGGAATGGTGACACGCCGTGCTATTACGGTACAGTACG
Internal bar code:ACGCTGACGGACTATAGACTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:304
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTTCTGTGCATGAGCCAT
Suggested primer 2:GGGCTTTCCTTCGAATCCCA