| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.078194 |
| Chromosome: | chromosome 11 |
| Location: | 3761726 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479200 | (1 of 5) PF00995 - Sec1 family (Sec1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCATACACACACAAATAACAAACACACACACACACACGCGCACGCAC |
| Internal bar code: | AGTACAAGTCTCTGCTATGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1259 |
| LEAP-Seq percent confirming: | 6.81818 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTGCCCTACCTGTCATT |
| Suggested primer 2: | AACTTGTCGCAGCAGATGGA |