Insertion junction: CLIP2.078196_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.078196
Chromosome:chromosome 10
Location:270355
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre10.g419500 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACTCCGGATGCTGCACCGCGCTGAAGCAGCCACCACAACTGAAGACCC
Internal bar code:TTCTACAATCTTGTTCAGTGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:967
LEAP-Seq percent confirming:30.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CAGAGCAGGCAACAAACCAC
Suggested primer 2:GGCTGTGGGATCTTCACGAA